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Update 'content/session/urinehormoneextractionaction.md'

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Tomislav Medak 2020-03-08 00:03:00 +00:00
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content/session/urinehormoneextractionaction.md
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@ -6,7 +6,7 @@ Using a basic understanding of chemistry principles such as polarity and solubil
The results of this short experiment will be a brown sticky substance that is a collection of all kinds of steroidal-like molecules, and will be followed by a reflective discussion: What kinds of queer, disobedient embodiments can we find in urinary hormones? How can we generate new subjectivities around hormones? Can we imagine speculative scenarios where we recycle hormones produced in the body? The results of this short experiment will be a brown sticky substance that is a collection of all kinds of steroidal-like molecules, and will be followed by a reflective discussion: What kinds of queer, disobedient embodiments can we find in urinary hormones? How can we generate new subjectivities around hormones? Can we imagine speculative scenarios where we recycle hormones produced in the body?
# Materials: # Materials
- Paper towels - Paper towels
- Scissors - Scissors
- Cups (for urine) - Cups (for urine)
@ -28,7 +28,7 @@ The results of this short experiment will be a brown sticky substance that is a
- Aquarium air pump with ¼” tubing - Aquarium air pump with ¼” tubing
- Pot for boiling - Pot for boiling
# DIY Column Construction: # DIY Column Construction
Two metal rods are fixed to a bracket by zip ties. Two metal rods are fixed to a bracket by zip ties.
One pipe clamp per metal rod is then fixed by zip ties. One pipe clamp per metal rod is then fixed by zip ties.
Make an incision around a glass bottle using the glass bottle cutter. Make an incision around a glass bottle using the glass bottle cutter.
@ -38,7 +38,7 @@ Poke a small hole with tweezers.
Pack column with a layer of cigarette filters (preferably to take off the wrapper). This will be the nonpolar environment. Pack column with a layer of cigarette filters (preferably to take off the wrapper). This will be the nonpolar environment.
Pack column with a layer of silica gel (amount depends on volume of bottle). This will be the polar environment. Pack column with a layer of silica gel (amount depends on volume of bottle). This will be the polar environment.
# Urine Extraction: # Urine Extraction
Collect a urine sample in a plastic cup. Collect a urine sample in a plastic cup.
Place a new plastic cup underneath the column (waste collection). Place a new plastic cup underneath the column (waste collection).
Pour methanol (5-10mL) down the column. This is the “conditioning” step. Pour methanol (5-10mL) down the column. This is the “conditioning” step.
@ -51,6 +51,6 @@ Place the falcon tube of methanol-hormones in a pot of boiling water. Use air pu
Once you see a dry, brown, sticky substance, you can take the tube off the hot water. The final product is a collection of hormones, or steroidal molecules in general. To isolate only estrogen would require an additional step of purification, which needs to be further investigated. Once you see a dry, brown, sticky substance, you can take the tube off the hot water. The final product is a collection of hormones, or steroidal molecules in general. To isolate only estrogen would require an additional step of purification, which needs to be further investigated.
Smell and share with your friends! Smell and share with your friends!
# Text Resources: # Text Resources
http://wlu18www30.webland.ch/wiki/Open_Source_Estrogen#Urine_Hormone_Extraction_Action http://wlu18www30.webland.ch/wiki/Open_Source_Estrogen#Urine_Hormone_Extraction_Action